اعلانات هامة

The present study demonstrates a procedure for the rapid development of a high number of somatic embryos from callus culture of Phoenix dactylifera L., cv. Zaghlool .

The calluses were induced from leaf segments as explants  subcultured on Murashige and Skoog medium supplemented with 1 and 10 mg L-1 picloram, 2,4-D, IBA  and NAA.

The results showed that the formation of callus was  significantly affected by type and concentrations of auxins . The highest percentage of callus formation from explants was induced by media supplemented with 10mg L-1 picloram (  46.3%  ) followed by 10mg L-12,4-D ( 28.3%  ) .

Somatic embryo  formation and development was achieved when calluses were transferred into  liquid and agar MS medium including  NAA at 0 . 1mg L-1 concentrations . In general, the growth of somatic embryos in liquid medium gave better results than solid medium with the same composition.

The highest number of pro-embryos ( 64.5 ) and the highest value of fresh weight of  differentiated cultures (2.97 g) were observed with liquid medium containing NAA.

Key words:, Phoenix dactylifera, leaf segments, somatic embryogenesis

أجريت هذه الدراسة لتحقيق إنتاج وتطور سريع لعدد كبير من الأجنة الجسدية من زراعة الكالوس في النخيل الثمري صنف ًزغلولً . يتم إنتاج الكالوس من خلال زراعة أجزاء من أوراق فتية على بيئة موراشي وسكوك لعام 1962 ( MS/1962  ) مع إضافة أنواع  مختلفة من الاوكسينات ( Pic – 2,4-D – NAA – IBA  ) بتراكيز ( 0 – 1 – 10 مغ/ ل ) .