اعلانات هامة

The aim of this study was to prepare a safe vaccine against brucellosis. For this purpose antigens were extracted by using acetic acid trichloride method, As well as antigen was prepared for brucillen test. The study reveals that the concentration of protein in vaccine antigens was 63.2 mg/dL and the SDS polyacrylamide gel electrophoresis showed presence of six protein bands in molecular weight between 21000-67000 Dalton . The extracted antigen used in vaccination of Guinea pigs and the results of vaccination revealed that the antibody titer range is 266 by tube agglutination test, while the result of brucellin test appeared that the range of

redness diameter is 14m and an increase in thickness of skin fold 2.08m after 24h from injection, while the range of redness diameter is 12m and increase in thickness of skin fold is 1.78m after 48h from injection, The challenge test revealed the ability of prepared antigen in protection of Guinea pigs against Brucella melitensis infection

هدفت هذه الدراسة إلى محاولة إنتاج لقاح آمن ضد مرض البروسيلات , ولهذا الغرض تم استخلاص مستضدات من البر وسيلة المالطية الذرية Rev1 باستخدام طريقة حمض الخل ثلاثي الكلور . كما تم تحضير مستضدات خاصة بفحص البر وسيلين من نفس الذرية لاستخدامه كأداة في مقايسة المناعة المتوسطة بالخلايا .